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Sample GSM379733 Query DataSets for GSM379733
Status Public on Jun 30, 2011
Title Patient6_339_GH_Day3_GH2_2PM (4 days)
Sample type RNA
 
Source name Milk Fat Globule
Organism Homo sapiens
Characteristics sample: Milk Fat Globule
Extracted molecule total RNA
Extraction protocol RNA was isolated from the milk fat globule using Trizol following the manufacturer's protocol
Label Biotin
Label protocol 440 ng total RNA was used for in vitro transcription, overnight for 14h using biotin-11-dUTP for labeling of the cRNA product using the Illumina®TotalPrep RNA amplification kit (Ambion, Inc., Austin, TX)
 
Hybridization protocol 750 ng of labeled cRNAs were hybridized to the Human Ref 8 V2 Beadchip arrays (Illumina Inc., San Diego, CA) at 55oC overnight (16h) following the Illumina Whole-Genome Gene Expression Protocol for BeadStation (Doc. # 111226048 Rev. B, Illumina Inc) and stained with 1 ug/ml streptavidin-Cy3 (Amersham Biosciences, Piscataway, NJ) for visualization
Scan protocol Probe arrays were scanned using the BeadArray reader (Factor 1)
Description Day1_GH0_5PM
Data processing Gene expression analysis was performed using the Sentrix BeadChip and BeadStation system from Illumina, Inc
 
Submission date Mar 12, 2009
Last update date Jun 30, 2011
Contact name Patricia Maningat
E-mail(s) [email protected]
Organization name Baylor College of Medicine
Department CNRC
Street address 1100 Bates St
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
 
Platform ID GPL6104
Series (1)
GSE15208 Gene Expression in the Human Mammary Epithelium during Lactation: the Milk Fat Globule Transcriptome through 4 Days

Data table header descriptions
ID_REF
VALUE cubic spline normalized signal intensity
Avg_NBEADS
BEAD_STDERR
Detection Pval

Data table
ID_REF VALUE Avg_NBEADS BEAD_STDERR Detection Pval
ILMN_1809034 127.3094 43 4.426308 0.007062147
ILMN_1660305 362.9969 36 12.92884 0
ILMN_1792173 203.4082 31 6.450577 0.001412429
ILMN_1762337 60.69265 45 2.323895 0.6723164
ILMN_1736007 64.67365 44 2.999396 0.440678
ILMN_1787689 73.7501 41 2.992811 0.1341808
ILMN_1731507 56.86697 33 3.290905 0.8700565
ILMN_1814316 72.83419 34 4.067255 0.1525424
ILMN_1745607 59.67796 40 2.216544 0.730226
ILMN_1757454 65.77562 46 2.33599 0.391243
ILMN_1735045 79.91063 37 4.010505 0.06779661
ILMN_1680754 80.40327 34 3.869156 0.06779661
ILMN_1659452 64.80227 37 2.685987 0.4350283
ILMN_1767388 156.3276 43 5.144844 0.002824859
ILMN_1673870 63.64298 40 1.841541 0.4887006
ILMN_1675204 63.59078 41 2.462169 0.4929379
ILMN_1755321 155.0713 47 6.18713 0.002824859
ILMN_1698554 1136.414 38 42.29187 0
ILMN_1805938 61.42242 49 2.73877 0.6299435
ILMN_1676336 80.6011 36 3.766107 0.06779661

Total number of rows: 22184

Table truncated, full table size 910 Kbytes.




Supplementary data files not provided
Processed data included within Sample table
Processed data provided as supplementary file
Processed data are available on Series record
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