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Sample GSM379749 Query DataSets for GSM379749
Status Public on Jun 30, 2011
Title Patient4_339_GH_Day1_GH0_2AM (4 days)
Sample type RNA
 
Source name Milk Fat Globule
Organism Homo sapiens
Characteristics sample: Milk Fat Globule
Extracted molecule total RNA
Extraction protocol RNA was isolated from the milk fat globule using Trizol following the manufacturer's protocol
Label Biotin
Label protocol 440 ng total RNA was used for in vitro transcription, overnight for 14h using biotin-11-dUTP for labeling of the cRNA product using the Illumina®TotalPrep RNA amplification kit (Ambion, Inc., Austin, TX)
 
Hybridization protocol 750 ng of labeled cRNAs were hybridized to the Human Ref 8 V2 Beadchip arrays (Illumina Inc., San Diego, CA) at 55oC overnight (16h) following the Illumina Whole-Genome Gene Expression Protocol for BeadStation (Doc. # 111226048 Rev. B, Illumina Inc) and stained with 1 ug/ml streptavidin-Cy3 (Amersham Biosciences, Piscataway, NJ) for visualization
Scan protocol Probe arrays were scanned using the BeadArray reader (Factor 1)
Description Day1_GH0_5PM
Data processing Gene expression analysis was performed using the Sentrix BeadChip and BeadStation system from Illumina, Inc
 
Submission date Mar 12, 2009
Last update date Jun 30, 2011
Contact name Patricia Maningat
E-mail(s) [email protected]
Organization name Baylor College of Medicine
Department CNRC
Street address 1100 Bates St
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
 
Platform ID GPL6104
Series (1)
GSE15208 Gene Expression in the Human Mammary Epithelium during Lactation: the Milk Fat Globule Transcriptome through 4 Days

Data table header descriptions
ID_REF
VALUE cubic spline normalized signal intensity
Avg_NBEADS
BEAD_STDERR
Detection Pval

Data table
ID_REF VALUE Avg_NBEADS BEAD_STDERR Detection Pval
ILMN_1809034 141.1021 58 3.812722 0.005649718
ILMN_1660305 481.7082 47 12.14558 0
ILMN_1792173 182.3804 46 7.081453 0.001412429
ILMN_1762337 65.07056 49 2.64949 0.4194915
ILMN_1736007 63.54278 45 2.707417 0.5
ILMN_1787689 75.91595 39 4.418235 0.08474576
ILMN_1731507 51.20514 39 2.445655 0.9872881
ILMN_1814316 66.72881 38 2.903069 0.3432204
ILMN_1745607 63.69087 37 3.37023 0.4915254
ILMN_1757454 60.46196 39 2.680149 0.6694915
ILMN_1735045 77.84877 37 5.274234 0.0720339
ILMN_1680754 71.00246 45 3.320863 0.180791
ILMN_1659452 60.21352 38 2.886205 0.6779661
ILMN_1767388 131.9651 39 6.256558 0.005649718
ILMN_1673870 62.36212 40 3.602834 0.5564972
ILMN_1675204 59.83348 49 2.246202 0.7048023
ILMN_1755321 139.4676 43 4.560031 0.005649718
ILMN_1698554 1303.083 48 27.88624 0
ILMN_1805938 58.04287 49 2.48427 0.789548
ILMN_1676336 81.34348 25 3.793207 0.04661017

Total number of rows: 22184

Table truncated, full table size 912 Kbytes.




Supplementary data files not provided
Processed data included within Sample table
Processed data provided as supplementary file
Processed data are available on Series record
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