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Sample GSM379783 Query DataSets for GSM379783
Status Public on Jun 30, 2011
Title Patient5_339_GH_Day2_GH1_11PM (4 days)
Sample type RNA
 
Source name Milk Fat Globule
Organism Homo sapiens
Characteristics sample: Milk Fat Globule
Extracted molecule total RNA
Extraction protocol RNA was isolated from the milk fat globule using Trizol following the manufacturer's protocol
Label Biotin
Label protocol 440 ng total RNA was used for in vitro transcription, overnight for 14h using biotin-11-dUTP for labeling of the cRNA product using the Illumina®TotalPrep RNA amplification kit (Ambion, Inc., Austin, TX)
 
Hybridization protocol 750 ng of labeled cRNAs were hybridized to the Human Ref 8 V2 Beadchip arrays (Illumina Inc., San Diego, CA) at 55oC overnight (16h) following the Illumina Whole-Genome Gene Expression Protocol for BeadStation (Doc. # 111226048 Rev. B, Illumina Inc) and stained with 1 ug/ml streptavidin-Cy3 (Amersham Biosciences, Piscataway, NJ) for visualization
Scan protocol Probe arrays were scanned using the BeadArray reader (Factor 1)
Description Day2_GH1_11PM
Data processing Gene expression analysis was performed using the Sentrix BeadChip and BeadStation system from Illumina, Inc
 
Submission date Mar 12, 2009
Last update date Jun 30, 2011
Contact name Patricia Maningat
E-mail(s) [email protected]
Organization name Baylor College of Medicine
Department CNRC
Street address 1100 Bates St
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
 
Platform ID GPL6104
Series (1)
GSE15208 Gene Expression in the Human Mammary Epithelium during Lactation: the Milk Fat Globule Transcriptome through 4 Days

Data table header descriptions
ID_REF
VALUE cubic spline normalized signal intensity
Avg_NBEADS
BEAD_STDERR
Detection Pval

Data table
ID_REF VALUE Avg_NBEADS BEAD_STDERR Detection Pval
ILMN_1809034 173.5265 40 5.012602 0.001412429
ILMN_1660305 300.5497 39 12.58421 0
ILMN_1792173 170.9837 30 5.633921 0.001412429
ILMN_1762337 67.43602 37 2.39692 0.2937853
ILMN_1736007 63.81135 38 3.410588 0.4943503
ILMN_1787689 71.76269 39 2.829785 0.1525424
ILMN_1731507 49.96093 39 2.320615 0.9929379
ILMN_1814316 68.58752 41 3.190276 0.2556497
ILMN_1745607 56.11906 41 2.222956 0.8855932
ILMN_1757454 67.0025 59 2.787632 0.3177966
ILMN_1735045 80.96284 49 3.745483 0.02824859
ILMN_1680754 81.11486 34 4.127074 0.02683616
ILMN_1659452 59.35661 27 3.733614 0.75
ILMN_1767388 98.04745 60 3.210176 0.01129944
ILMN_1673870 66.21876 31 4.050064 0.3601695
ILMN_1675204 59.58357 27 3.278644 0.7415254
ILMN_1755321 167.5318 43 5.181791 0.001412429
ILMN_1698554 1058.939 44 31.6654 0
ILMN_1805938 63.51713 41 2.833866 0.519774
ILMN_1676336 85.20072 45 2.746847 0.01553672

Total number of rows: 22184

Table truncated, full table size 905 Kbytes.




Supplementary data files not provided
Processed data included within Sample table
Processed data provided as supplementary file
Processed data are available on Series record

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