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Sample GSM379854 Query DataSets for GSM379854
Status Public on Jun 30, 2011
Title Patient2_339_GH_Day4_GH3_8AM (4 days)
Sample type RNA
 
Source name Milk Fat Globule
Organism Homo sapiens
Characteristics sample: Milk Fat Globule
Extracted molecule total RNA
Extraction protocol RNA was isolated from the milk fat globule using Trizol following the manufacturer's protocol
Label Biotin
Label protocol 440 ng total RNA was used for in vitro transcription, overnight for 14h using biotin-11-dUTP for labeling of the cRNA product using the Illumina®TotalPrep RNA amplification kit (Ambion, Inc., Austin, TX)
 
Hybridization protocol 750 ng of labeled cRNAs were hybridized to the Human Ref 8 V2 Beadchip arrays (Illumina Inc., San Diego, CA) at 55oC overnight (16h) following the Illumina Whole-Genome Gene Expression Protocol for BeadStation (Doc. # 111226048 Rev. B, Illumina Inc) and stained with 1 ug/ml streptavidin-Cy3 (Amersham Biosciences, Piscataway, NJ) for visualization
Scan protocol Probe arrays were scanned using the BeadArray reader (Factor 1)
Description Day4_GH3_8AMa
Data processing Gene expression analysis was performed using the Sentrix BeadChip and BeadStation system from Illumina, Inc
 
Submission date Mar 12, 2009
Last update date Jun 30, 2011
Contact name Patricia Maningat
E-mail(s) [email protected]
Organization name Baylor College of Medicine
Department CNRC
Street address 1100 Bates St
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
 
Platform ID GPL6104
Series (1)
GSE15208 Gene Expression in the Human Mammary Epithelium during Lactation: the Milk Fat Globule Transcriptome through 4 Days

Data table header descriptions
ID_REF
VALUE cubic spline normalized signal intensity
Avg_NBEADS
BEAD_STDERR
Detection Pval

Data table
ID_REF VALUE Avg_NBEADS BEAD_STDERR Detection Pval
ILMN_1809034 185.5344 40 6.34516 0.001412429
ILMN_1660305 424.529 35 12.41859 0
ILMN_1792173 174.5767 33 6.824985 0.001412429
ILMN_1762337 64.18893 52 2.720865 0.4322034
ILMN_1736007 63.61956 37 2.380772 0.4661017
ILMN_1787689 69.87103 38 3.224155 0.2090396
ILMN_1731507 56.32202 41 2.335257 0.8742938
ILMN_1814316 68.89982 37 3.153032 0.2457627
ILMN_1745607 62.89142 39 2.560303 0.5155367
ILMN_1757454 67.18507 33 3.200956 0.3121469
ILMN_1735045 76.01942 47 3.771648 0.07344633
ILMN_1680754 74.78524 46 3.039405 0.0960452
ILMN_1659452 58.95467 39 2.012505 0.7556497
ILMN_1767388 141.5936 44 6.497175 0.002824859
ILMN_1673870 67.52998 43 2.739951 0.2980226
ILMN_1675204 61.12031 50 2.386407 0.6172317
ILMN_1755321 127.1489 42 4.622964 0.004237288
ILMN_1698554 1680.057 43 47.35765 0
ILMN_1805938 59.37679 35 3.008326 0.730226
ILMN_1676336 84.67295 47 2.737991 0.02966102

Total number of rows: 22184

Table truncated, full table size 911 Kbytes.




Supplementary data files not provided
Processed data included within Sample table
Processed data provided as supplementary file
Processed data are available on Series record
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