|
| Status |
Public on May 13, 2009 |
| Title |
Cardiac Progenitor WT2 |
| Sample type |
RNA |
| |
|
| Source name |
Embryonic Cardiac Progenitors - wild type
|
| Organism |
Mus musculus |
| Characteristics |
cell type: E9.0 embryonic cardiac progenitors
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction
|
| Label |
Biotin
|
| Label protocol |
Total RNA was amplified with the WT-Ovation Pico RNA Amplification System, fragmented and labeled with the FL-Ovation cDNA Biotin Module V2 (Nugen).
|
| |
|
| Hybridization protocol |
Performed as manufacturer's protocol (Affymetrics)
|
| Scan protocol |
Performed as manufacturer's protocol (Affymetrics)
|
| Description |
YFP+ cells marking precardiac mesoderm were FACS-sorted (w/wo stabilized Beta-catenin), and their total RNA was isolated, amplified and labeled with the NUGENE pico kit for microarray analyses. Mutant samples were obtained from YFP+ cells isolated from embryos with Isl1-cre, Rosa-YFP, Beta-catenin (loxP ex3). The mutants are conditional knockout mouse embryos that activates stable Beta-catenin in cardiac progenitors.
|
| Data processing |
Raw data generated from at least three independent experiments were further analyzed by the group of Dr. Ru-Fang Yeh at the Center for Informatics and Molecular Biostatistics, UCSF.
|
| |
|
| Submission date |
Mar 13, 2009 |
| Last update date |
Mar 16, 2009 |
| Contact name |
Chulan Kwon |
| E-mail(s) |
[email protected]
|
| Organization name |
Gladstone Institutes
|
| Street address |
1650 Owens Street
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94158 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE15232 |
A Regulatory Pathway Involving Notch1/β-Catenin/Isl1 Determines Cardiac Progenitor Cell Fate |
|
