|
| Status |
Public on Aug 24, 2009 |
| Title |
Infected pft1 leaf, rep4 |
| Sample type |
RNA |
| |
|
| Source name |
pft1 leaves, inoculation with F. oxysporum
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
genotype: pft1 mutant
tissue: leaf
treatment: F. oxysporum
|
| Treatment protocol |
Treatment involved gently uprooting the plants from the soil, rinsing the roots with distilled water to remove excess soil and then incubating in either a F. oxysporum spore suspension (1 million spores/ml resuspended in water) or distilled water (mock) for 15 seconds before replanting in soil. Plants were moved to hotter, long day conditions (16hr light, 28C and 21C day night cycle) to promote infection and harvested 48 hours after treatment.
|
| Growth protocol |
Plants were grown under short day conditions (8hrs light, 24C and 21C day night cycle) in plastic trays (30 cells 5cmx5cm) with two plants per cell for 4 weeks before treatment.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy Plant Mini Kit (Qiagen) according to the manfacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
The RNA was labelled, hybridized, washed and scanned by the AGRF (Australian Genome Research Facility - http://www.agrf.org.au) according to Affymetrix requirements.
|
| |
|
| Hybridization protocol |
The RNA was labelled, hybridized, washed and scanned by the AGRF (Australian Genome Research Facility - http://www.agrf.org.au) according to Affymetrix requirements.
|
| Scan protocol |
The RNA was labelled, hybridized, washed and scanned by the AGRF (Australian Genome Research Facility - http://www.agrf.org.au) according to Affymetrix requirements.
|
| Description |
Expression data from pft1 leaves harvested 48 hours after inoculation with F. oxysporum.
|
| Data processing |
The data was analyzed using GenespringGX 7.3.1. The raw CEL files were pre-processed using the RMA algorithm and the resulting expression values were normalized per chip to the median across all chips.
|
| |
|
| Submission date |
Mar 15, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Brendan Kidd |
| E-mail(s) |
[email protected]
|
| Organization name |
CSIRO
|
| Department |
Plant Industry
|
| Street address |
306 Carmody Road
|
| City |
St Lucia |
| State/province |
QLD |
| ZIP/Postal code |
4067 |
| Country |
Australia |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE15236 |
Expression profiling of the Arabidopsis Mediator complex mutant pft1/med25 and wildtype infected with Fusarium oxysporum |
|
| Relations |
| Reanalyzed by |
GSE118579 |
| Reanalyzed by |
GSE119083 |
