|
| Status |
Public on Apr 17, 2009 |
| Title |
parental A2780 (cisplatin-sensitive), biological rep5 |
| Sample type |
RNA |
| |
|
| Source name |
parental A2780 cells (GI50 = 5uM)
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: parental A2780
|
| Growth protocol |
Cells were maintained in RPMI 1640 media with 2 mM L-glutamine, 50 U/ml penicillin, 50 mg/ml streptomycin, and 10% fetal bovine serum, at 30o C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human U133 plus 2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from parental A2780 cells
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed median target intensity of each array was arbitrarily set to 1000.
|
| |
|
| Submission date |
Mar 24, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Meng Li |
| E-mail(s) |
[email protected]
|
| Organization name |
Indiana University
|
| Department |
Medical Sciences
|
| Street address |
1001 E 3rd Street
|
| City |
Bloomington |
| State/province |
IN |
| ZIP/Postal code |
47405 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE15372 |
Expression data from A2780 (cisplatin-sensitive) and Round5 A2780 (cisplatin-resistant) cell lines. |
| GSE15709 |
Expression and methylation data from A2780 (cisplatin-sensitive) and Round5 A2780 (cisplatin-resistant) cell lines |
|
| Relations |
| Reanalyzed by |
GSE119087 |
