|
| Status |
Public on Jun 12, 2019 |
| Title |
Liver, control F1_rep2 |
| Sample type |
SRA |
| |
|
| Source name |
liver
|
| Organism |
Mus musculus |
| Characteristics |
strain: ICR
tissue: liver of F1 males
age: 8 wk of age
stress: control
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Livers were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent.
Libraries were constructed with TruSeq DNA library preparation kit (Illumina)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
| |
|
| Description |
C6
|
| Data processing |
Reads of RNA-seq were mapped to the mouse genome (mm10) with STAR
Mapped reads were subsequently assembled into transcripts guided by the reference annotation (GENCODE gene models) with Stringtie
Gene differential expression analysis using DESeq2 based on the reads count file obtained by HTSeq
Genome_build: mm10
Supplementary_files_format_and_content: counts
|
| |
|
| Submission date |
Jun 11, 2019 |
| Last update date |
Jun 12, 2019 |
| Contact name |
Jing-Jing Zhang |
| E-mail(s) |
[email protected]
|
| Organization name |
Huazhong Agricultural University
|
| Department |
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction
|
| Lab |
Miaolab
|
| Street address |
1 Lion Rock Street
|
| City |
Wuhan |
| State/province |
Hubei |
| ZIP/Postal code |
430070 |
| Country |
China |
| |
|
| Platform ID |
GPL21273 |
| Series (2) |
| GSE132521 |
Paternal overdiet drives intergenerational transmission of metabolic disorders through sperm epigenetic alteration [RNA-Seq] |
| GSE132613 |
Paternal overdiet drives intergenerational transmission of metabolic disorders through sperm epigenetic alteration |
|
| Relations |
| BioSample |
SAMN12015899 |
| SRA |
SRX6035987 |
