|
Status |
Public on Apr 01, 2009 |
Title |
RAW_0.35mM_HOCl_#1 |
Sample type |
RNA |
|
|
Source name |
RNA from RAW 264.7 Cells
|
Organism |
Mus musculus |
Characteristics |
Array Type; Affymetrix Mouse 430_2 amount: 5ug bioanalyzer results: Good cell type: RAW 264.7
|
Biomaterial provider |
Courtney G. Woods, The Hamner Institutes, 6 Davis Dr, RTP, NC 27709, [email protected], 919-558-1267, 919-558-1300
|
Treatment protocol |
Treatment: 0.35 mM HOCl in mediu Medium: see growth protocol below Treatment Duration: 6 hrs Cell Confluency upon Treatement: ~80-90%
|
Growth protocol |
RAW 264.7 macrophages (RAW Cells) were cultured in DMEM supplemented with 10% FBS, 10mM HEPES, 100 U penicillin/ml, and 100 ug streptomycin/ml
|
Extracted molecule |
total RNA |
Extraction protocol |
Triazol
|
Label |
biotin
|
Label protocol |
standard Affymetrix protocol
|
|
|
Hybridization protocol |
standard Affymetrix protocol
|
Scan protocol |
standard Affymetrix protocol
|
Description |
no additional information
|
Data processing |
Robust Multi-array Average
|
|
|
Submission date |
Mar 30, 2009 |
Last update date |
Mar 31, 2009 |
Contact name |
Courtney G Woods |
E-mail(s) |
[email protected]
|
Phone |
919-558-1267
|
Fax |
919-558-1300
|
Organization name |
The Hamner Institutes
|
Department |
Computational Biology
|
Street address |
PO Box 12137, 6 Davis Drive
|
City |
Research Triangle Park |
State/province |
NC |
ZIP/Postal code |
27709 |
Country |
USA |
|
|
Platform ID |
GPL1261 |
Series (1) |
GSE15457 |
Gene Expression Profiling of Cellular Dose-Response to Hypochlorous Acid (HOCl) |
|