|
| Status |
Public on Apr 01, 2009 |
| Title |
control replicon expressing S2 cells |
| Sample type |
RNA |
| |
|
| Source name |
Drosophila S2 cells, mock infected
|
| Organism |
Drosophila melanogaster |
| Characteristics |
cell line: S2.pF1fs
virus: control plasmid
infection: mock infected
|
| Treatment protocol |
Cells were induced with 1 mM copper sulfate for 18 h prior to RNA extraction.
|
| Growth protocol |
Cells cultured at 25C in Schneider's Drosophila media supplemented with 10% heat-inactivated fetal bovine serum, 10 U/ml penicillin, and 10 ug/ml streptomycin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA done per the manufacturer's instruction. Total RNA digested with RQ1 DNAse and reisolated with RNAsy columns per the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNAs were prepared from 5-10 ug total RNA using standard Affymetix protocol.
|
| |
|
| Hybridization protocol |
Fragmented cRNAs were hybridized to Affymetrix Drosophila genome array chips for 16 h at 45C, washed, and stained using an Affymetrix microfluids station.
|
| Scan protocol |
Chips were scanned with an Affymetrix Scanner 3000.
|
| Description |
Miller S2 FHV replicon control
|
| Data processing |
The data were analyzed using the Affymetrix package of Bioconductor - expression values were calculated using a robust multi-array average (RMA).
|
| |
|
| Submission date |
Mar 31, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
David Miller |
| E-mail(s) |
[email protected]
|
| Phone |
734-763-0565
|
| Organization name |
University of Michigan
|
| Department |
Internal Medicine
|
| Street address |
3560B MSRB II, 1150 W. Medical Center Dr.
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109-5688 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (2) |
| GSE15467 |
Transcriptional response of Drosophila cells to FHV RNA1 replicon expression (replicon experiment) |
| GSE15469 |
Transcriptional response of Drosophila cells to FHV RNA replication |
|
| Relations |
| Reanalyzed by |
GSE119084 |
