|
| Status |
Public on Jul 22, 2009 |
| Title |
PPRF-ILC-day4_2 |
| Sample type |
RNA |
| |
|
| Source name |
re-differentiated cells, PPRF protocol, day 4
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: re-differentiated cells after expansion from islets
|
| Treatment protocol |
INGAP 1.0 ug/mL
|
| Growth protocol |
INGAP 1.0 ug/mL
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen
|
| Label |
biotin
|
| Label protocol |
Affymetrix
|
| |
|
| Hybridization protocol |
Following fragmentation, ten micrograms of adjusted cRNA from each sample was hybridized for 16 hours at 45 deg C to Affymetrix GeneChip
|
| Scan protocol |
Scanning was performed using the Affymetrix GeneChip 3000 Scanner. Images were processed into CEL files with the Affymetrix GCOS software.
|
| Description |
Re-differentiated cells after expansion from islets with PPRF protocol day 4
|
| Data processing |
Raw CEL intensity data were RMA normalized using R/Bioconductor
|
| |
|
| Submission date |
Apr 01, 2009 |
| Last update date |
Jul 22, 2009 |
| Contact name |
Burak Kutlu |
| E-mail(s) |
[email protected]
|
| Phone |
206-732-1200
|
| Organization name |
Institute for Systems Biology
|
| Street address |
1441 North 34th Street
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98103 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE15543 |
Meta analysis of gene expression in human islets after in vitro expansion. |
|
