|
| Status |
Public on Jun 01, 2009 |
| Title |
Rat_Tracheal_Dex&Smoke_2hr, rep6 |
| Sample type |
RNA |
| |
|
| Source name |
Tracheal explants, Dex&Smoke, 2hr
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Sprague-Dawley
tissue: tracheal explants
treatment: dexamethasone & cigarette smoke at 2hrs
replicate: 6
|
| Treatment protocol |
Control (air), smoke (10 puffs of cigarette smoke, delivered during 15 mins), dexamethasone (1 x 10-7M) and dexamethasone + cigarette smoke.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the NuGEN RNA Amplification System V2 protocol from 50 ug total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on Rat 230_2 whole Genome Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
|
| Description |
DS6_2h_35
Gene expression data from rat tracheal explants exposed to dexamethasone & cigarette smoke (treatment) at 2hrs.
|
| Data processing |
The data was analysed with Rosetta Resolver version 5.0.
|
| |
|
| Submission date |
Apr 06, 2009 |
| Last update date |
Sep 19, 2011 |
| Contact name |
Darren Gormley |
| E-mail(s) |
[email protected]
|
| Organization name |
GlaxoSmithKline
|
| Street address |
Gunnelswood Road
|
| City |
Stevenage |
| ZIP/Postal code |
SG1 2NY |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE15563 |
Modifications of the Rat Airway Explant Transcriptome by Cigarette Smoke |
|
