|
| Status |
Public on Jul 31, 2024 |
| Title |
MDA-MB-231_TCI04 treated_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
MDA-MB-231, 50 μM TCI04 treated, 48h
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MDA-MB-231 cell line
agent: TCI04
|
| Treatment protocol |
MDA-MB-231 cells were treated with 50 μM of TCI04 or PBS for 48 h.
|
| Growth protocol |
MDA-MB-231 cells were cultured in DMEM with 10% FBS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from 50 μM TCI04 treated MDA-MB-231 cells
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Jun 20, 2019 |
| Last update date |
Jul 31, 2024 |
| Contact name |
Ping Zhou |
| E-mail(s) |
[email protected]
|
| Phone |
13821755783
|
| Organization name |
Tianjin Medical University Cancer Institute and Hospotal
|
| Street address |
No1 Huanhuxi Load
|
| City |
Tianjin |
| State/province |
Tianjin |
| ZIP/Postal code |
300070 |
| Country |
China |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE133049 |
Gene expression data after treated with TCI04 in MDA-MB-231 cells |
|
