|
| Status |
Public on Jun 21, 2019 |
| Title |
BS_NS_BAL_708 |
| Sample type |
genomic |
| |
|
| Source name |
Bronchoalveolar lavage cells from healthy individual
|
| Organism |
Homo sapiens |
| Characteristics |
Sex: M
age: 28
smoking_status: Nonsmoker
pack_years: NA
cig_daily: NA NA
fvc: 108
fev1: 104
fev1_fvc: 81
percent_recovery_bal: 62
total_cells_bal_millions: 24.3
cell_conc_bal_millions_pr_litre: 159.7
percent_macrophages: 93.4
percent_lymphocytes: 4.2
percent_neutrophils: 2.2
percent_eosinophils: 0.2
percent_basophils: 0
mast_cells: 1
cd4_cd8_ratio: 6.4
subject_id: BAL_708
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was extracted using AllPrep DNA/RNA/miRNA Universal Kit (Qiagen) according to the manufacturer’s protocol
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
Conventional bisulfite conversion as well as oxidative bisulfite conversion using TrueMethyl conversion kit from Cambridge Epigenetix (CEGX). 250 ng of DNA per reaction followed by llumina MethylationEPIC BeadChip hybridization via Standard Illumina Protocol
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina HiScan
|
| Description |
Sample_8
|
| Data processing |
SQN normalisation
|
| |
|
| Submission date |
Jun 20, 2019 |
| Last update date |
Jun 21, 2019 |
| Contact name |
Mikael Ringh |
| Organization name |
Karolinska Institutet
|
| Street address |
Medical Epigenetics, CMM L8:00, Karolinska University Hospital Solna
|
| City |
Stockholm |
| State/province |
Choose state (US & Canada) |
| ZIP/Postal code |
17176 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL23976 |
| Series (1) |
| GSE133062 |
Tobacco smoking induces changes in true DNA methylation, hydroxymethylation and gene expression in bronchoalveolar lavage cells |
|
