|
| Status |
Public on Sep 04, 2009 |
| Title |
90' PDS 2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
M. tuberculosis delta-sigmaH mutant at log phase + 1 hr diamide 10 mM, then washed away + 90 min
|
| Organism |
Mycobacterium tuberculosis |
| Characteristics |
genotype/variation: delta-sigmaH mutant
protocol: log phase + 1 hr diamide 10 mM, then washed away + 90 min
|
| Treatment protocol |
Mehra S & Kaushal D. J. Bacteriol. In Press.
|
| Growth protocol |
M. tuberculosis and its isogenic mutant were grown in Middlebrook's 7H9 supplemented media. Specific manipulations are mentioned in Mehra S & Kaushal D. J. Bacteriol. In Press.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol/Beadbeater method using a Biospec Beadbeater and 0.1 mm silica/zirconia beads
|
| Label |
Cy3
|
| Label protocol |
ftp://ftp.jcvi.org/pub/data/PFGRC/pdf_files/protocols/M007.pdf
|
| |
|
| Channel 2 |
| Source name |
M. tuberculosis at log phase + 1 hr diamide 10 mM, then washed away + 90 min
|
| Organism |
Mycobacterium tuberculosis |
| Characteristics |
genotype/variation: wild type
protocol: log phase + 1 hr diamide 10 mM, then washed away + 90 min
|
| Treatment protocol |
Mehra S & Kaushal D. J. Bacteriol. In Press.
|
| Growth protocol |
M. tuberculosis and its isogenic mutant were grown in Middlebrook's 7H9 supplemented media. Specific manipulations are mentioned in Mehra S & Kaushal D. J. Bacteriol. In Press.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol/Beadbeater method using a Biospec Beadbeater and 0.1 mm silica/zirconia beads
|
| Label |
Cy5
|
| Label protocol |
ftp://ftp.jcvi.org/pub/data/PFGRC/pdf_files/protocols/M007.pdf
|
| |
|
| |
| Hybridization protocol |
ftp://ftp.jcvi.org/pub/data/PFGRC/pdf_files/protocols/M008.pdf
|
| Scan protocol |
scanned on an Axon 4000B scanner using GenePix 6.1
|
| Description |
Biological replicate 2 , 90' post treatment
|
| Data processing |
LOWESS normalized, background subtracted data obtained from log2 of processed Red signal/processed Green signal, using S+ ArrayAnalyzer/Spotfire
|
| |
|
| Submission date |
Apr 08, 2009 |
| Last update date |
Sep 04, 2009 |
| Contact name |
Deepak Kaushal |
| E-mail(s) |
[email protected]
|
| Organization name |
Tulane National Primate Research Center
|
| Department |
Bacteriology
|
| Lab |
Kaushal
|
| Street address |
18703 Three Rivers Rd
|
| City |
Covington |
| State/province |
LA |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
|
| Platform ID |
GPL8382 |
| Series (1) |
| GSE15608 |
Functional Genomics Reveals Extended Roles of the Mycobacterium tuberculosis Stress Response Factor sigmaH |
|
