|
| Status |
Public on Oct 08, 2019 |
| Title |
PG_Mor_R4_C3 |
| Sample type |
SRA |
| |
|
| Source name |
Embryo
|
| Organism |
Homo sapiens |
| Characteristics |
embryo type: PG embryo
developmental stage: morula
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Single-cell poly-A(+) RNA was reverse transcribed and amplified by Smart-seq2 protocol
Single-cell libraries were constructed using Nextera XT DNA Library Preparation Kit (Illumina Cat#FC-131-1096)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
| |
|
| Data processing |
quality control using Trimmomatic version 0.35
clean reads were mapped to the human reference genome of GRCh38 using STAR version v 2.5.2b aligne
uniquely mapped read counts of each annotated Ensemble gene were calculated by HTSeq (v0.9.1)
Genome_build: GRCh38
Supplementary_files_format_and_content: tab-delimited text files include TPM values for each sample.
|
| |
|
| Submission date |
Jul 05, 2019 |
| Last update date |
Oct 08, 2019 |
| Contact name |
leng lizhi |
| E-mail(s) |
[email protected]
|
| Organization name |
Central South University
|
| Street address |
xiangya road
|
| City |
Changsha |
| ZIP/Postal code |
410008 |
| Country |
China |
| |
|
| Platform ID |
GPL20795 |
| Series (2) |
| GSE133854 |
Single-cell transcriptome analysis of uniparental embryos reveals parent-of-origin effects on human preimplantation development [RNA-seq] |
| GSE133856 |
Single-cell transcriptome analysis of uniparental embryos reveals parent-of-origin effects on human preimplantation development |
|
| Relations |
| BioSample |
SAMN12219471 |
| SRA |
SRX6407901 |
