tissue: blood cell type: natural killer cells gender: male
Treatment protocol
w/o recombinant IFNa-2b
Growth protocol
6h RPMI, 10% human AB serum, 100 U/ml penicillin, 100 µg/ml streptomycin, 37°C, 5%CO2
Extracted molecule
total RNA
Extraction protocol
RNAeasy
Label
biotin
Label protocol
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol
Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at conditions recommended by the manufacturer. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol
GeneChips were scanned using the Affymetrix GCS 3000.
Description
Cultured for 6h without Interferon alpha.
Data processing
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
