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Sample GSM394095 Query DataSets for GSM394095
Status Public on Aug 18, 2010
Title 6h High Interferon alpha MHH1
Sample type RNA
 
Source name PBMC, 5 male healthy humans
Organism Homo sapiens
Characteristics tissue: blood
cell type: natural killer cells
gender: male
treatment: recombinant IFNa-2b
dosage: 100 ng/ml
Treatment protocol 100 ng/ml recombinant IFNa-2b
Growth protocol 6h RPMI, 10% human AB serum, 100 U/ml penicillin, 100 µg/ml streptomycin, 37°C, 5%CO2
Extracted molecule total RNA
Extraction protocol RNAeasy
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at conditions recommended by the manufacturer. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Affymetrix GCS 3000.
Description Cultured for 6h with high dosage of Interferon alpha.
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
 
Submission date Apr 20, 2009
Last update date Aug 21, 2009
Contact name Robert Geffers
E-mail(s) [email protected]
Phone +49 531-6181-3058
Organization name HCI - Helmholtz Centre for Infection Research
Department Dep. Molecular Bacteriology
Lab Genome Analytics
Street address Inhoffenstr. 7
City Braunschweig
ZIP/Postal code 38124
Country Germany
 
Platform ID GPL570
Series (1)
GSE15743 IFN alpha-induced gene expression in human NK cells

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
1007_s_at 111.8 P 0.007543
1053_at 30.9 P 0.017001
117_at 97.8 P 0.008689
121_at 194.2 P 0.02786
1255_g_at 11.9 A 0.418069
1294_at 1596.9 P 0.000219
1316_at 74.4 P 0.013092
1320_at 3.6 A 0.939581
1405_i_at 5114.9 P 0.000491
1431_at 17.1 A 0.127645
1438_at 5.5 A 0.92617
1487_at 184 P 0.039365
1494_f_at 36.6 A 0.162935
1552256_a_at 33.6 A 0.27417
1552257_a_at 140.1 P 0.046143
1552258_at 16.7 A 0.27417
1552261_at 52.8 A 0.067627
1552263_at 882.9 P 0.000244
1552264_a_at 355.5 P 0.001221
1552266_at 16 A 0.303711

Total number of rows: 54675

Table truncated, full table size 1415 Kbytes.




Supplementary file Size Download File type/resource
GSM394095.CEL.gz 5.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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