|
| Status |
Public on Aug 01, 2009 |
| Title |
Mid-third_instar_larval_eye_disc_GMR-upd_rep5 |
| Sample type |
RNA |
| |
|
| Source name |
Total RNA, single Drosophila mid-third instar larval eye disc, GMR-upd
|
| Organism |
Drosophila melanogaster |
| Characteristics |
genotype: Glass multiple reporter unpaired (GMR-upd)
protocol: Arcturus Isolation kit (PicoPure RNA Isolation Kit)
|
| Treatment protocol |
single Drosophila mid-third instar larval eye disc were dissected from equally aged larvae (2-hr time collections)
|
| Growth protocol |
N/A
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Arcturus Isolation kit (PicoPure RNA Isolation Kit) was used to extract total cellular RNA according to the manufacturer's protocol, and the RNA was amplified and converted into cDNA using the Arcturus Amplification kit (RiboAmp® RNA Amplification Kit)
|
| Label |
biotin
|
| Label protocol |
Labeled anti-sense RNA (aRNA) was synthesized from the resulting cDNA using the ENZO BioArrayTM High Yield RNA Transcript Labeling Kit.
|
| |
|
| Hybridization protocol |
9 mcg of amplified and biotin-labeled control (yw) and GMR-upd aRNA were separately hybridized for 16 hrs onto the GeneChip Drosophila Genome 2.0 Arrays (Affymetrix)
|
| Scan protocol |
Affymetrix 7G GeneArray scanner
|
| Description |
Gene expression data from a single Drosophila mid-third instar larval eye disc
|
| Data processing |
Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the MBEI algorithm using dChip program. No further adjustments were made to the data in the table.
|
| |
|
| Submission date |
Apr 28, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Jiri ZAVADIL |
| E-mail(s) |
[email protected]
|
| Phone |
+33648065733
|
| Organization name |
IARC WHO
|
| Street address |
150 COURS ALBERT THOMAS
|
| City |
LYON |
| State/province |
Other |
| ZIP/Postal code |
69008 |
| Country |
France |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE15868 |
Expression profiling in the Drosophila eye reveals unexpected repression of Notch signaling by the JAK/STAT pathway |
|
| Relations |
| Reanalyzed by |
GSE119084 |
