|
| Status |
Public on Aug 07, 2009 |
| Title |
Oxidative stress, t45 min |
| Sample type |
RNA |
| |
|
| Source name |
H2O2 treated S. cerevisiae cells
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain: 4741
stress: oxidative stress
time: 45 min
|
| Treatment protocol |
At time 0 cells were treated with mild stresses and then transftered to another stress
|
| Growth protocol |
Cells were grown in YPD to mid log phase in 30 degrees
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using MasterPure™ (EPICENTER Biotechnologies)
|
| Label |
biotin
|
| Label protocol |
cDNA was prepared using poly(T) primers, labeled cRNA was prepared according to standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
According to standard Affymetrix protocol
|
| Scan protocol |
According to standard Affymetrix protocol
|
| Description |
Gene expression in H2O2 0.66mM, 45min
|
| Data processing |
RMA algorithms using bioconductor software implemented in R
|
| |
|
| Submission date |
May 03, 2009 |
| Last update date |
Aug 07, 2009 |
| Contact name |
Amir Mitchell |
| E-mail(s) |
[email protected]
|
| Organization name |
Weizmann Inst.
|
| Street address |
Hertzel
|
| City |
Rehovot |
| ZIP/Postal code |
76100 |
| Country |
Israel |
| |
|
| Platform ID |
GPL2529 |
| Series (1) |
| GSE15936 |
Expression data from S. cerevisiae under serial stresses |
|
