|
| Status |
Public on May 26, 2020 |
| Title |
wt_ctl_bm1 |
| Sample type |
SRA |
| |
|
| Source name |
bone marrow neutrophil
|
| Organism |
Mus musculus |
| Characteristics |
strain/background: C57BL/6
cell type: Bone marrow neutrophil
condition: Steady state
sorting: Sorted by cKit+ Gr1+
|
| Extracted molecule |
total RNA |
| Extraction protocol |
10x Genomics Single Cell 3’ Library Kit V2.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
Single cell RNA-seq
|
| Data processing |
Sequenced reads of mouse were aligned to the mm10 mouse transcriptome, then quantify the expression of transcripts in each cell using Cell Ranger v2.2.0.
Sequenced reads of human were aligned to the hg38 human transcriptome, then quantify the expression of transcripts in each cell using BD™ Rhapsody Whole Transcriptome Assay Analysis Pipeline.
Genome_build: mm10 (mouse) or hg38 (human)
Supplementary_files_format_and_content: *.txt: Text files include UMIs for each Sample.
|
| |
|
| Submission date |
Sep 16, 2019 |
| Last update date |
May 27, 2020 |
| Contact name |
Xuemei Xie |
| E-mail(s) |
[email protected]
|
| Organization name |
Boston Children's Hospital
|
| Department |
Transfusion Medicine
|
| Lab |
Luo's Lab
|
| Street address |
811 Enders Building, 320 Longwood Ave.
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL24247 |
| Series (2) |
| GSE137539 |
Single-cell transcriptome profiling reveals neutrophil heterogeneity in homeostasis and infection |
| GSE137540 |
Single-cell transcriptome profiling reveals neutrophil heterogeneity in homeostasis and infection |
|
| Relations |
| BioSample |
SAMN12769555 |
| SRA |
SRX6855648 |
