|
| Status |
Public on Apr 28, 2020 |
| Title |
Paeruginosa_PAO1_EmptyVector_2 |
| Sample type |
SRA |
| |
|
| Source name |
bacterial culture
|
| Organism |
Pseudomonas aeruginosa PAO1 |
| Characteristics |
strain: PAO1
genotype/variation: Empty vector
tissue: bacterial culture
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
Bacteria were grown on swarm plates, and the swarm fronts were harvested and suspended in RNA-Protect Bacteria Reagent
|
| Data processing |
Illumina software used for basecalling.
Sequence reads were mapped to the pseudomonas aeruginosa genome using STAR v2.6.
Count data was generated from STAR produced BAM files using HTSeq v0.61p1.
|
| |
|
| Submission date |
Sep 19, 2019 |
| Last update date |
Apr 28, 2020 |
| Contact name |
Shannon Coleman |
| Organization name |
University of British Columbia
|
| Street address |
2259 Lower Mall
|
| City |
Vancouver |
| State/province |
BC |
| ZIP/Postal code |
V6T 1Z4 |
| Country |
Canada |
| |
|
| Platform ID |
GPL18782 |
| Series (1) |
| GSE137738 |
Overexpression of the small RNA PA0805.1 modulates the expression of a large set of genes and proteins, resulting in altered motility, cytotoxicity and tobramycin resistance |
|
| Relations |
| BioSample |
SAMN12788784 |
| SRA |
SRX6874326 |
