|
Status |
Public on Apr 28, 2020 |
Title |
Paeruginosa_PAO1_PA08051_1 |
Sample type |
SRA |
|
|
Source name |
bacterial culture
|
Organism |
Pseudomonas aeruginosa PAO1 |
Characteristics |
strain: PAO1 genotype/variation: PA0805.1 over-expression tissue: bacterial culture
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA libraries were prepared for sequencing using standard Illumina protocols
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2500 |
|
|
Description |
Bacteria were grown on swarm plates, and the swarm fronts were harvested and suspended in RNA-Protect Bacteria Reagent
|
Data processing |
Illumina software used for basecalling. Sequence reads were mapped to the pseudomonas aeruginosa genome using STAR v2.6. Count data was generated from STAR produced BAM files using HTSeq v0.61p1.
|
|
|
Submission date |
Sep 19, 2019 |
Last update date |
Apr 28, 2020 |
Contact name |
Shannon Coleman |
Organization name |
University of British Columbia
|
Street address |
2259 Lower Mall
|
City |
Vancouver |
State/province |
BC |
ZIP/Postal code |
V6T 1Z4 |
Country |
Canada |
|
|
Platform ID |
GPL18782 |
Series (1) |
GSE137738 |
Overexpression of the small RNA PA0805.1 modulates the expression of a large set of genes and proteins, resulting in altered motility, cytotoxicity and tobramycin resistance |
|
Relations |
BioSample |
SAMN12788812 |
SRA |
SRX6874330 |