|
| Status |
Public on Dec 21, 2009 |
| Title |
MEF2-HS+LPS |
| Sample type |
RNA |
| |
|
| Source name |
Mouse embryo fibroblasts #2
|
| Organism |
Mus musculus |
| Characteristics |
strain: ICR
tissue: Embryo
developmental stage: Embryonic day 15.5
stress: heat shock at 42˚C for 1 hour
agent: LPS for 4 hours after HS pretreatment
|
| Treatment protocol |
Cells were maintained at 37˚C (Control), treated with lipopolysaccharide (LPS, E. coli 0127:B8, Sigma, 1 μg/ml) for 4 hours, or treated with LPS for 4 hours after the pretreatment of heat shock at 42˚C for 1 hour.
|
| Growth protocol |
Cells were maintained at 37˚C in 5% CO2 in Dulbecco’s modified eagle medium (DMEM) containing 10% fetal bovine serum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared using RNeasy Mini (Qiagen).
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared from 1 μg total RNA with a One-Cycle cDNA Synthesis Kit and 3'-Amplification Reagents for IVT Labeling (Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 μg of cRNA were hybridized for 16 hr at 45˚C on GeneChip Mouse Genome 430 2.0 Array (Affymetrix). Arrays were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
Arrays were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
Gene expression data from cells treated with LPS for 4 hours after the pretreatment of heat shock at 42˚C for 1 hour
|
| Data processing |
The data were analyzed with GeneChip Operating Softwere version 1.4 (GCOS1.4, Affymetrix) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
May 28, 2009 |
| Last update date |
Jan 10, 2011 |
| Contact name |
Akira Nakai |
| E-mail(s) |
[email protected]
|
| Phone |
81-836-22-2214
|
| Fax |
81-836-22-2315
|
| Organization name |
Yamaguchi University
|
| Department |
Graduate School of Medicine
|
| Lab |
Dept. of Biochemistry and Molecular Biology
|
| Street address |
Minami-Kogushi 1-1-1
|
| City |
Ube |
| State/province |
Yamaguchi Prefecture |
| ZIP/Postal code |
755-8505 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE16266 |
Identification of inflammatory genes suppressed by heat shock |
|
