|
| Status |
Public on Mar 23, 2020 |
| Title |
A318 bud 1000 chill hours rep1 |
| Sample type |
SRA |
| |
|
| Source name |
floral bud
|
| Organism |
Prunus persica |
| Characteristics |
tissue: floral bud
Stage: 1000 CH
cultivar number: A318
|
| Growth protocol |
Apricot trees were grown in France. Peach trees were grown in South Carolina, USA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from apricot bud tissue were extracted using NuceloSpin RNA XS Isolation kit (Macherey-Nagel, France). Total RNA from peach tissue were extracted using ??? Kit
Library construction was followed the illumina TruSeq RNA Library Prep Kit
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
Adaptors and low-quality reads were trimmed by skewer for each library
Trimmed reads were aligned to Prunus persica v1 and Prunus persica v2 by STAR-2.5.3a
Gene counts were quantified by HTSeq-count
Gene counts of Prupe.1G531100 to Prupe.1G531700 (Pp01:43417246...43480648) in the Purnus persica v2 were replaced by ppa018667m, ppb017585m, ppa010758m, ppa011123m, ppa010822m, and ppa010714m in Prunus persica v1
Genome_build: Prunus persica v2 and Prunus persica v1
Supplementary_files_format_and_content: comma-delimited files with gene counts for apricot and peach
|
| |
|
| Submission date |
Oct 11, 2019 |
| Last update date |
Mar 23, 2020 |
| Contact name |
Margaret Staton |
| E-mail(s) |
[email protected], [email protected]
|
| Phone |
8645064515
|
| Organization name |
University of Tennessee
|
| Street address |
2505 EJ Chapman Blvd, 370 PBB
|
| City |
Knoxville |
| State/province |
Tennessee |
| ZIP/Postal code |
37996 |
| Country |
USA |
| |
|
| Platform ID |
GPL21993 |
| Series (1) |
| GSE138792 |
Abiotic stress response and adaptive phenology in fruit trees |
|
| Relations |
| BioSample |
SAMN13020271 |
| SRA |
SRX6983191 |
