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| Status |
Public on Oct 24, 2019 |
| Title |
CFSEpos_D20 |
| Sample type |
SRA |
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| Source name |
PBMC
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| Organism |
Macaca mulatta |
| Characteristics |
cell type: PBMC
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| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were cryopreserved, sorted using FACS and captured using 10x genomics single cell platform
10x single cell 3' v2
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
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| Description |
PBMC were cryopreserved on date indicated. Cells were thawed on December 8, 2017. Cells were enriched for CD4+ using immunomagnetic kit and sorted for CFSE+ (D20)
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| Data processing |
Cellranger 2.1.1 default-parameters
Monocle
Genome_build: Mmul8
Supplementary_files_format_and_content: tab-delim file of genes(rows) and cells (columns)
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| Submission date |
Oct 21, 2019 |
| Last update date |
Oct 24, 2019 |
| Contact name |
Geoff R Hill |
| Organization name |
Fred Hutchinson Cancer Research Center
|
| Department |
CRD
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| Lab |
Hill Lab
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| Street address |
1100 Fairview Ave. N.
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| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109 |
| Country |
USA |
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| Platform ID |
GPL21120 |
| Series (1) |
| GSE139200 |
Single cell RNAseq of adoptively transferred Tregs in rhesus macaques |
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| Relations |
| BioSample |
SAMN13022999 |
| SRA |
SRX6985757 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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