|
| Status |
Public on Mar 26, 2011 |
| Title |
AFI027 |
| Sample type |
RNA |
| |
|
| Source name |
Scrub typhus
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: PBMCs
disease group: Scrub typhus
age (years): 19
gender: Male
fever day: 5
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality of RNA was evaluated by gel electrophoresis.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Illumina® TotalPrep RNA Amplification Kit
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
AFI027
|
| Data processing |
Using GeneSpring GX9 software, quantile normalization was applied to raw signal intensities (non-normalized data) exported from BeadStudio software; then baseline transformation to median of normal control samples was performed.
|
| |
|
| Submission date |
Jun 05, 2009 |
| Last update date |
Mar 26, 2011 |
| Contact name |
Thanavadee Prachason |
| E-mail(s) |
[email protected]
|
| Organization name |
Mahidol University
|
| Street address |
Division of Molecular Genetics, 10th floor Adulyadejvikrom Bld., Siriraj Hospital, Bangkoknoi
|
| City |
BKK |
| ZIP/Postal code |
10700 |
| Country |
Thailand |
| |
|
| Platform ID |
GPL6102 |
| Series (1) |
| GSE16463 |
Orientsia tsutsugamushi stimulates an original gene expression program in monocytes: relationship with gene expression in patients with scrub typhus |
|
