|
| Status |
Public on Apr 12, 2005 |
| Title |
MIN6 growth at 5.5 mM of glucose and treated at 20 uM of amylin during 2h |
| Sample type |
RNA |
| |
|
| Source name |
MIN6, 5.5 mM of glucose,treated at 20 uM of amylin during 2h
|
| Organism |
Mus musculus |
| Treatment protocol |
Treated at 20 uM of amylin during 2h
|
| Growth protocol |
Murine pancreatic beta cell line MIN6 was growth at 5.5 mM of glucose, 37 ºC, 5% CO2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
According to the protocol enclosed with the RNeasy Mini Kit (Qiagen, Hilden, Germany)
|
| Label protocol |
According to Affymetrix protocol (biotin)
|
| |
|
| Hybridization protocol |
According to Affymetrix protocol (biotin labeled cRNA)
|
| Scan protocol |
According to Affymetrix protocol
|
| Description |
Murine pancreatic beta cell line MIN6 was growth at 5.5 mM of glucose, 37 ºC, 5% CO2 and was treated at 20 uM of human amylin during 2h
|
| Data processing |
Raw data was background adjusted, normalized and summarized by RMA (Affy package version 1.12.2, R version 2.4.0)
|
| |
|
| Submission date |
Feb 08, 2005 |
| Last update date |
Feb 01, 2007 |
| Contact name |
Silvia Casas Fontdevila |
| E-mail(s) |
[email protected]
|
| Phone |
0034932275400-2910
|
| Fax |
0034934515272
|
| URL |
http://www.idibaps.ub.edu/eng/home.php
|
| Organization name |
IDIBAPS
|
| Lab |
Laboratory of Experimental Diabetes
|
| Street address |
Villarroel, 170
|
| City |
Barcelona |
| ZIP/Postal code |
08036 |
| Country |
Spain |
| |
|
| Platform ID |
GPL339 |
| Series (1) |
| GSE2253 |
Beta cells (MIN6) treated with amylin at different times and doses and growth at different concentrations of glucose |
|
