NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM414943 Query DataSets for GSM414943
Status Public on Jun 10, 2009
Title Pancreatic Sample 17-Tumor
Sample type RNA
 
Source name PancreaticSample17-Tumor
Organism Homo sapiens
Characteristics tissue: Tumor Tissue in Pancreatic Cancer Sample
sex: Female
age: 76
Extracted molecule total RNA
Extraction protocol RNA extraction was performed as Affymetrix GeneChip expression technical manual (Affymetrix, Inc., Santa Clara, CA). Briefly, total RNA samples were extracted, followed by measurement of the A2260/a280 ratio with at least 1.8 for pure RNA. Quality of the RNA was checked by an Agilent 2100 Bioanalyzer.  The Bioanalyzer gel profile exhibited a 28S band that is 2 times more intense than 18S ribosomal RNA. The quality of RNA was assessed by agarose gel electrophoresis.
Label biotin
Label protocol Lableing was performed according to Affymetrix Gene Chip technical manual
 
Hybridization protocol The targets for Affymetrix DNA microarray analysis were prepared according to the manufacturer’s instructions. Biotin-labeled cRNA, produced by in vitro transcription, was fragmented and hybridized to Affymetrix GeneChip Human Genome U133 Plus 2.0 Arrays at 45°C for 16 hr and then washed and stained using the GeneChip Fluidics.
Scan protocol The arrays were scanned by a GeneArray Scanner and patterns of hybridization detected as light emitted from the fluorescent reporter groups incorporated into the target and hybridized to oligonucleotide probes.
Description Gene expression of -PancreaticSample17-Tumor
Data processing The data were analyzed with Agilent Gene Spring GX 7.3 version using Affymetrix default analysis settings and GC-RMA as normalization method.
 
Submission date Jun 09, 2009
Last update date Aug 28, 2018
Contact name Liewei Wang
E-mail(s) [email protected]
Phone 507-284-5264
Fax 507-284-4455
URL http://mayoresearch.mayo.edu/staff/wang_l2.cfm
Organization name Mayo Clinic
Department Molecular Pharmacology and Experimental Therapeutics
Street address 200 First street SW
City Rochester
State/province MN
ZIP/Postal code 55905
Country USA
 
Platform ID GPL570
Series (1)
GSE16515 Expression data from Mayo Clinic Pancreatic Tumor and Normal samples
Relations
Reanalyzed by GSE119087

Data table header descriptions
ID_REF
VALUE GCRMA normalized signal intensity

Data table
ID_REF VALUE
1007_s_at 621.50
1053_at 76.56
117_at 1007.00
121_at 53.92
1255_g_at 4.99
1294_at 116.40
1316_at 16.06
1320_at 9.74
1405_i_at 924.50
1431_at 16.30
1438_at 10.67
1487_at 79.49
1494_f_at 9.26
1552256_a_at 129.80
1552257_a_at 146.40
1552258_at 21.16
1552261_at 8.57
1552263_at 73.88
1552264_a_at 72.14
1552266_at 16.52

Total number of rows: 54613

Table truncated, full table size 890 Kbytes.




Supplementary file Size Download File type/resource
GSM414943.cel.gz 4.4 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap