|
Status |
Public on Sep 16, 2009 |
Title |
breast cancer sample from patient BC61 |
Sample type |
genomic |
|
|
Source name |
patient BC61
|
Organism |
Homo sapiens |
Characteristics |
histological grade: 3 tnm: pT2pN2pMX er: NA pr: NA her2: NA invasion: yes node: positive histopathology type: infiltrating ductal carcinoma
|
Treatment protocol |
None
|
Growth protocol |
None
|
Extracted molecule |
genomic DNA |
Extraction protocol |
DNA was extracted from tumor samples using the QIAamp DNA mini kit (Qiagen, Inc., Valencia, CA, USA).
|
Label |
Biotin
|
Label protocol |
As per manufacturer (Affymetrix)
|
|
|
Hybridization protocol |
As per manufacturer (Affymetrix)
|
Scan protocol |
Arrays were washed and stained in the Genechip Fluidics Station 450 (Affymetrix) following the manufacturer’s instructions. Chips were scanned with the Affymetrix GeneChip Scanner 3000.
|
Description |
Hybridized to SNP5.0 primary data: Affymetrix *.CHP files for the 250K_Nsp and 250K_Sty microarrays; Affymetrix *.CHP files for the SNP5.0 microarray.
|
Data processing |
*.CHP files for the 250K_Nsp and 250K_Sty experiments were generated using the Affymetrix Power Tools version 1.8.5 gtype-probeset-genotype program. *.CHP files for the SNP5.0 experiments were generated using the Affymetrix Power Tools version 1.10.0 apt-probeset-genotype program.
|
|
|
Submission date |
Jun 13, 2009 |
Last update date |
Sep 16, 2009 |
Contact name |
Robert J Clifford |
E-mail(s) |
[email protected]
|
Phone |
301-435-1527
|
Fax |
301-480-6641
|
Organization name |
NCI
|
Department |
CCR
|
Lab |
LPG
|
Street address |
2115 E Jefferson
|
City |
Rockville |
State/province |
MD |
ZIP/Postal code |
20850 |
Country |
USA |
|
|
Platform ID |
GPL6804 |
Series (1) |
GSE16619 |
Identification of novel gene amplification events in breast cancer |
|