|
| Status |
Public on Jun 28, 2010 |
| Title |
ea_control2_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
eye-antennal imaginal discs atoGal4 (NP6558)
|
| Organism |
Drosophila melanogaster |
| Characteristics |
genotype/variation: control
replicate: 1
line: atoGal4
developmental stage: third instar larva
tissue: eye-antennal imaginal discs
|
| Biomaterial provider |
Laboratory of Neurogenetics, VIB-KULeuven
|
| Growth protocol |
Flies and crosses are kept in standard food at 18 degrees, at early third instar stage shifted for 24 hours at 28 degrees Celcius
|
| Extracted molecule |
total RNA |
| Extraction protocol |
eye-antennal imaginal discs are dissected from 3rd instar larvae in RNAlater (Ambion) and transferred to the RNA extraction buffer, on ice, of the ZymoResearch mini RNA isolation kit.
|
| Label |
biotin
|
| Label protocol |
RNA concentration and purity were determined spectrophotometrically using the Nanodrop ND-1000 (Nanodrop Technolgies) and RNA integrity was assessed using a Bioanalyser 2100 (Agilent). Per sample, an amount of 2 µg of total RNA spiked with four bacterial RNA transcripts (Affymetrix) was converted and amplified to double stranded cDNA in a 1-cycle cDNA reverse transcription reaction. Subsequently the sample was converted to antisense cRNA and labeled with biotin through an in vitro transcription reaction according to the manufacturers protocol (Affymetrix). All amplification and labeling reactions were performed on a Biomek 3000 ArrayPlex Workstation (Beckman Coulter).
|
| |
|
| Hybridization protocol |
A mixture of purified and fragmented biotinylated cRNA and added hybridisation controls (Affymetrix) was hybridised on Affymetrix Drosophila 2.0 arrays followed by staining and washing in the GeneChip® fluidics station 450 (Affymetrix) according to the manufacturer’s procedures.
|
| Scan protocol |
To assess the raw probe signal intensities, chips were scanned using the GeneChip scanner 3000 (Affymetrix).
|
| Description |
eye-antennal imaginal discs of third instar larvae of Drosophila melanogaster
|
| Data processing |
RMA
|
| |
|
| Submission date |
Jun 19, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Rekin's Janky |
| E-mail(s) |
[email protected]
|
| Organization name |
VIB
|
| Department |
Nucleomics Core
|
| Street address |
Herestraat 49 Box 816
|
| City |
Leuven |
| ZIP/Postal code |
B-3000 |
| Country |
Belgium |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE16713 |
Atonal and Senseless perturbations in Drosophila melanogaster eye-antennal imaginal discs. |
|
| Relations |
| Reanalyzed by |
GSE119084 |
