|
| Status |
Public on Jan 09, 2020 |
| Title |
day 21 -3 |
| Sample type |
RNA |
| |
|
| Source name |
Whole blood
|
| Organism |
Mus musculus |
| Characteristics |
day: 21
agent: Mycobacterium tuberculosis H37Rv
strain: BALB
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Blood was collected in Tempus reagent (Life Technologies) at 1:2 ratio. Total RNA was extracted using the PerfectPure RNA Blood Kit (5 PRIME). Globin RNA was depleted from total RNA (1.5–2 µg) using the Mouse GLOBINclear kit (Ambion).
|
| Label |
biotin
|
| Label protocol |
cRNA was prepared from 200 ng globin-reduced blood RNA using the Illumina TotalPrep RNA Amplification Kit (Ambion). Quality was checked using an RNA 6000 Nano kit (Agilent) using a BioAnalyzer 2100 (Agilent). Biotinylated cRNA samples were randomized.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Data processing |
raw illumina text files were read into R (v3.5) and normalised using the neqc function from limma package (v3.38.3), with default parameters
|
| |
|
| Submission date |
Nov 25, 2019 |
| Last update date |
Jan 10, 2020 |
| Contact name |
Anne OGarra |
| E-mail(s) |
[email protected]
|
| Organization name |
Francis Crick Insititue
|
| Lab |
IMMUNOREGULATION AND INFECTION LABORATORY
|
| Street address |
1 Midland Road
|
| City |
London |
| ZIP/Postal code |
NW1 1AT |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL6887 |
| Series (2) |
| GSE140943 |
Mouse transcriptome reveals potential signatures of protection and pathogenesis in human tuberculosis [blood array] |
| GSE140945 |
Mouse transcriptome reveals signatures of protection and pathogenesis in human tuberculosis |
|
