|
| Status |
Public on Oct 02, 2009 |
| Title |
High Translation Fraction 0.25% YE-1 |
| Sample type |
RNA |
| |
|
| Source name |
Whole 7-8 old male
|
| Organism |
Drosophila melanogaster |
| Characteristics |
strain: w1118
gender: male
diet: 6 days on 0.25% YE food
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was separated over a sucrose gradient, then RNA was isolated by Trizol extraction from the high or the low translation fractions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard protocol for MessageAmp II-Biotin Enhanced kit (Ambion) starting with 100 ng total RNA.
|
| |
|
| Hybridization protocol |
12.5 micrograms of biotin-labeled cRNA was fragmented and hybridized according to the Affymetrix protocol.
|
| Scan protocol |
Arrays were scanned using the Affymetrix GeneChip 3000 scanner.
|
| Description |
Expression profile of the high translation fraction on 0.25%YE
|
| Data processing |
Data were processed using Rosetta Resolver (Rosetta Biosoftware); the Affymetrix-Rosetta – Intensity Profile and Experiment Builder pipelines were used. Data processing resulted in average expression values and associated errors as well as a statistic reporting whether the target transcript was detected above background.
|
| |
|
| Submission date |
Jun 22, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Brian Matthew Zid |
| E-mail(s) |
[email protected]
|
| Organization name |
University of California San Diego
|
| Department |
Chemistry and Biochemistry
|
| Lab |
Zid
|
| Street address |
9500 Gilman Drive
|
| City |
La Jolla |
| State/province |
California |
| ZIP/Postal code |
92093 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE16738 |
Translational Changes Upon Dietary Restriction in Drosophila |
|
| Relations |
| Reanalyzed by |
GSE119084 |
