|
| Status |
Public on Mar 07, 2022 |
| Title |
3mo BUMC M WT 3 |
| Sample type |
RNA |
| |
|
| Source name |
humerus
|
| Organism |
Mus musculus |
| Characteristics |
tissue: humerus
animal: 286
batch: batch 2
colony: BUMC
timepoint: 3 months
Sex: male
brd2 genotype: wt/wt
|
| Growth protocol |
Animals were allowed to age for 3 to 18 months and were maintained under standard feeding and light dark cycle conditions at BUMC.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was purified from whole mouse humerus flash frozen under liquid nitrogen and stored at -80C until processed for total RNA. Total bones were powdered with a mortar and pestle under liquid nitrogen and placed in Qiagen Tissue Lyzer tubes with 1 ml of Qiazol reagent per humerus. After extraction aqueous solutions for the Qiazol were applied to a miRNA RNeasy column (catalog #217004).
|
| Label |
biotin
|
| Label protocol |
Biotin labeling was performed using the Ambion WT Expression Kit (Life Technologies, Grand Island, NY) according to the manufacturer's protocol, followed by the GeneChip WT Terminal Labeling and Controls Kit (Thermo Fisher, Waltham, MA).
|
| |
|
| Hybridization protocol |
The labeled, fragmented DNA was hybridized to the Affymetrix Mouse Gene 1.0 ST Array for 18 hours in a GeneChip Hybridization oven 640 at 45oC with rotation (60 rpm). The hybridized samples were washed and stained using an Affymetrix fluidics station 450.
|
| Scan protocol |
After staining, microarrays were immediately scanned using an Affymetrix GeneArray Scanner 3000 7G Plus.
|
| Description |
Gene expression data from humerus of BUMC male Brd2wt/wt animal at 3 months.
|
| Data processing |
Raw Affymetrix CEL files were normalized to produce Entrez Gene-identifier-specific expression values using the implementation of the Robust Multiarray Average (RMA) in the affy Bioconductor R package and the Brainarray mogene10stv1mmentrezgcdf R package (version 14.0.0).
|
| |
|
| Submission date |
Dec 04, 2019 |
| Last update date |
Mar 09, 2022 |
| Contact name |
Boston University Microarray and Sequencing Resource |
| E-mail(s) |
[email protected]
|
| Organization name |
Boston University
|
| Department |
Microarray and Sequencing Resource
|
| Street address |
72 East Concord Street, E631
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02118 |
| Country |
USA |
| |
|
| Platform ID |
GPL13730 |
| Series (1) |
| GSE141451 |
Temporal and Quantitative Transcriptomic Differences Define Sexual Dimorphism in Murine Post Natal Bone Aging |
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