NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM422111 Query DataSets for GSM422111
Status Public on Aug 31, 2009
Title Peripheral blood monocytes, CD16 negative, rep3
Sample type RNA
 
Source name peripheral blood monocytes, CD16-
Organism Homo sapiens
Characteristics donor health: healthy
tissue: peripheral blood
cell type: monocyte
cd16: negative
Treatment protocol ex vivo
Growth protocol ex vivo
Extracted molecule total RNA
Extraction protocol Total RNA from Mo pellets was isolated by Trizol extraction and purified using RNeasy columns (Qiagen). The quality of RNA was assessed by visualization of intact bands corresponding to 18S and 28S rRNA on formaldehyde agarose gels.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2003, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array (Platform ID: GPL570). GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 2500A.
Description Gene expression data ex vivo.
021904_CD16_negative
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date Jun 25, 2009
Last update date Aug 28, 2018
Contact name Petronela ANCUTA
E-mail(s) [email protected]
Phone 5148908000
Fax 514-412-7377
Organization name Université de Montréal
Department Microbiology and Immunology
Lab Chemokines and HIV
Street address 264, BVD Rene-Levesque East
City Montreal
State/province Quebec
ZIP/Postal code H2X 1P1
Country Canada
 
Platform ID GPL570
Series (1)
GSE16836 Transcriptional profiling of CD16+ and CD16- peripheral blood monocytes from healthy individuals
Relations
Reanalyzed by GSE49910
Reanalyzed by GSE86362
Reanalyzed by GSE119087

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 1742.48 P 0.000509415
AFFX-BioB-M_at 3506.42 P 4.42873e-05
AFFX-BioB-3_at 1482.87 P 0.000224668
AFFX-BioC-5_at 5211.15 P 5.16732e-05
AFFX-BioC-3_at 5072.41 P 4.42873e-05
AFFX-BioDn-5_at 5091.04 P 4.42873e-05
AFFX-BioDn-3_at 23905.3 P 5.16732e-05
AFFX-CreX-5_at 67455.1 P 5.16732e-05
AFFX-CreX-3_at 82256.5 P 4.42873e-05
AFFX-DapX-5_at 3886.11 P 4.42873e-05
AFFX-DapX-M_at 6357.09 P 0.000509415
AFFX-DapX-3_at 7334.19 P 4.42873e-05
AFFX-LysX-5_at 321.333 P 0.00499819
AFFX-LysX-M_at 694.315 P 0.0284573
AFFX-LysX-3_at 681.028 P 5.16732e-05
AFFX-PheX-5_at 405.508 P 0.000224668
AFFX-PheX-M_at 734.841 P 0.000581214
AFFX-PheX-3_at 692.01 P 0.00179591
AFFX-ThrX-5_at 950.082 P 0.00159257
AFFX-ThrX-M_at 1343.35 P 8.14279e-05

Total number of rows: 54675

Table truncated, full table size 1611 Kbytes.




Supplementary file Size Download File type/resource
GSM422111.CEL.gz 4.5 Mb (ftp)(http) CEL
GSM422111.CHP.gz 6.4 Mb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap