|
| Status |
Public on Aug 31, 2009 |
| Title |
Peripheral blood monocytes, CD16 negative, rep4 |
| Sample type |
RNA |
| |
|
| Source name |
peripheral blood monocytes, CD16-
|
| Organism |
Homo sapiens |
| Characteristics |
donor health: healthy
tissue: peripheral blood
cell type: monocyte
cd16: negative
|
| Treatment protocol |
ex vivo
|
| Growth protocol |
ex vivo
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from Mo pellets was isolated by Trizol extraction and purified using RNeasy columns (Qiagen). The quality of RNA was assessed by visualization of intact bands corresponding to 18S and 28S rRNA on formaldehyde agarose gels.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2003, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array (Platform ID: GPL570). GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 2500A.
|
| Description |
Gene expression data ex vivo.
042304_CD16_negative
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Jun 25, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Petronela ANCUTA |
| E-mail(s) |
[email protected]
|
| Phone |
5148908000
|
| Fax |
514-412-7377
|
| Organization name |
Université de Montréal
|
| Department |
Microbiology and Immunology
|
| Lab |
Chemokines and HIV
|
| Street address |
264, BVD Rene-Levesque East
|
| City |
Montreal |
| State/province |
Quebec |
| ZIP/Postal code |
H2X 1P1 |
| Country |
Canada |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE16836 |
Transcriptional profiling of CD16+ and CD16- peripheral blood monocytes from healthy individuals |
|
| Relations |
| Reanalyzed by |
GSE49910 |
| Reanalyzed by |
GSE86362 |
| Reanalyzed by |
GSE119087 |
