|
| Status |
Public on Dec 01, 2022 |
| Title |
Mannitol rep03 |
| Sample type |
RNA |
| |
|
| Source name |
adult head, 7 days of treatment
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: head
diet fed: Mannitol
genotype: Oregon R
gender: male
age: 10 days
Stage: adult
|
| Treatment protocol |
At the end of treatment, flies were anesthetized using CO2 and collected in Trizol.
|
| Growth protocol |
30 adult unmated males Oregon R flies per vial fed respective diet for 7 days at 25C temperature.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions (RNeasy Microarray Tissue Handbook) .
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 250 ng total RNA (GeneChip 3’ IVT Express Kit).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
Gene expression data using Affymetrix Drosophila Genome 2.0 array
|
| Data processing |
The data was analysed using R/Bioconductor affy and limma packages. Quantile Normalization and RMA background correction was used.
|
| |
|
| Submission date |
Dec 19, 2019 |
| Last update date |
Dec 01, 2022 |
| Contact name |
Manoj Ramesh Teltumbade |
| E-mail(s) |
[email protected]
|
| Organization name |
Institute of Genomics and Integrative Biology
|
| Lab |
Drosophila Lab
|
| Street address |
Mathura Road, Near Sukhdev Vihar, bus depo
|
| City |
New Delhi |
| State/province |
Delhi |
| ZIP/Postal code |
110025 |
| Country |
India |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE142384 |
Drug screening in Drosophila for repurposing in neuropsychiatry |
|
