|
| Status |
Public on Aug 31, 2020 |
| Title |
lncRNA profile of case482T |
| Sample type |
RNA |
| |
|
| Source name |
non-small-cell lung cancer
|
| Organism |
Homo sapiens |
| Characteristics |
status after 3 years: Alive
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using an acid phenol/chloroform extraction method followed by ethanol precipitation.
|
| Label |
Cy5
|
| Label protocol |
2.5 μg of total RNA of intrahepatic cholangiocarcinoma/normal intrahepatic bile duct tissue was employed with 100 nmol/L of pCp-DY647 (Dharmacon, Lafayette, CO, USA) and 15 Units of T4 RNA ligase (USB, Cleveland, Oh, USA) in a total reaction volume of 20 µL at 16°C overnight
|
| |
|
| Hybridization protocol |
The labeled RNAs were hybridized to the array with a 1×hybridization solution (5× Denhart’s solution, 0.5% SDS, 5× SSC) in a Hybridization Chamber (Corning Inc, Corning, NY, USA) at 46°C for 12 to 16 hours.
|
| Scan protocol |
Scanned in a LuxScanTM -10K Scanner (capitalBio Inc.,Beijing, China).
|
| Description |
The microarray was used to acquire lncRNA expression profiles of 2412 lncRNAs.
|
| Data processing |
Quantile normalization across multiple arrays,GPR normailization software, log 2 transformation of expression levels was used.
|
| |
|
| Submission date |
Jan 03, 2020 |
| Last update date |
Aug 31, 2020 |
| Contact name |
Rui-qi WANG |
| E-mail(s) |
[email protected]
|
| Phone |
18670518621
|
| Organization name |
SUN YAN-SEN UNIVERSITY CANCER CENTER
|
| Department |
state key lab
|
| Lab |
726
|
| Street address |
651 DONGFENG EAST ROAD, GUANGZHOU 510060, P. R. CH
|
| City |
GZ |
| State/province |
Guangdong |
| ZIP/Postal code |
510060 |
| Country |
China |
| |
|
| Platform ID |
GPL27968 |
| Series (1) |
| GSE143018 |
Identification of a Four-lncRNA Signature Predicting Prognosis of Patients with NSCLC |
|
