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Sample GSM4251741 Query DataSets for GSM4251741
Status Public on Aug 18, 2021
Title liver, Tg, 6487
Sample type SRA
 
Source name liver
Organism Mus musculus
Characteristics genetic background: Creld2 eGFP/eGFP
treatment: Tg
tissue: liver
Extracted molecule total RNA
Extraction protocol RNA was isolated using the Nucleospin RNA II kit (Macherey & Nagel) according to the manufacturer’s instructions
According to the manufacturer´s instructions in the Tru-Seq RNA library preparation kit v2, 10 ng of RNA was converted into cDNA libraries
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 1500
 
Description Pharmacological in vivo model for non-alcoholic fatty liver induction
Drug-induced ER stress
Data processing base calling and de-multipexing using CASAVA version 1.8
Alignment with TopHat2 v2.0.11 and SAMtools version 1.4 using the default parameters
Quantification with Partek Genomics Suite V6.6
reads mapped to each transcript against the RefSeq Hg19 annotation download on May 2015
Normalization using DeSeq2
Genome_build: hg19
Supplementary_files_format_and_content: Comma separated file includes DeSeq2 rlog counts for each Sample.
 
Submission date Jan 06, 2020
Last update date Aug 18, 2021
Contact name Elvira Mass
E-mail(s) [email protected]
Organization name LIMES
Street address Carl-Troll str
City Bonn
ZIP/Postal code 53115
Country Germany
 
Platform ID GPL18480
Series (1)
GSE143185 Creld2 function during unfolded protein response is essential for liver metabolism homeostasis
Relations
BioSample SAMN13740135
SRA SRX7506325

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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