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Sample GSM426497 Query DataSets for GSM426497
Status Public on Nov 19, 2009
Title Reference 3hpi 4
Sample type RNA
 
Source name wounded inflorescence stalk
Organism Arabidopsis thaliana
Characteristics ecotype: Ws-2
tissue: wounded inflorescence stalk segments
inoculant: mock
Treatment protocol The base of the first emerging main inflorescence stalk (3-5 cm) of intact plants was wounded with a syringe needle, mock-inoculated and grown for another three hours.
Growth protocol Arabidopsis thaliana plants were cultivated in a growth chamber under short-day conditions (8 h of light, 120 µE) at 22°C and 16°C during the dark period (16 h).
Extracted molecule total RNA
Extraction protocol Total RNA was extracted, including digestion of DNA, from plant tissue samples by using the RNeasy Plant Mini Kit according to manufacturer’s protocol (Qiagen, Hilden, Germany).
Label biotin
Label protocol Affymetrix protocol.
 
Hybridization protocol Affymetrix protocol.
Scan protocol The GeneChip® Scanner 3000 7G is also controlled by GeneChip® Operating Software(GCOS).
Description Gene expression data of reference tissue were compared with those of inoculated tissue.
Data processing VSN (variance stabilization normalization, reference: Huber et al. (2002) Bioinformatics 18: suppl. 1, S96-S104)
 
Submission date Jul 10, 2009
Last update date Aug 15, 2018
Contact name Rosalia Deeken
E-mail(s) [email protected]
Phone +49 931 8886121
Fax +49 931 8886158
Organization name University of Wuerzburg, Julius-von-Sachs-Institute
Department Botany I; Plant Molecular Physiology & Biophysics
Lab Prof. Hedrich
Street address Julius-von-Sachs-Platz 2
City Wuerzburg
ZIP/Postal code 97072
Country Germany
 
Platform ID GPL198
Series (2)
GSE13929 Arabidopsis thaliana three hours after infection with Agrobacterium tumefaciens
GSE14106 Transcriptome data from inflorescence stalks of intact A. thaliana plants after infection with A. tumefaciens
Relations
Reanalyzed by GSE118579

Data table header descriptions
ID_REF
VALUE log2 expression value

Data table
ID_REF VALUE
244901_at 7.08883
244902_at 7.15736
244903_at 8.02058
244904_at 7.56067
244905_at 6.85665
244906_at 7.35826
244907_at 6.85202
244908_at 6.8151
244909_at 6.71391
244910_s_at 6.66994
244911_at 6.56587
244912_at 10.5707
244913_at 7.09815
244914_at 6.87207
244915_s_at 6.99618
244916_at 6.75059
244917_at 6.73191
244918_at 6.6282
244919_at 6.998
244920_s_at 8.59015

Total number of rows: 22810

Table truncated, full table size 401 Kbytes.




Supplementary file Size Download File type/resource
GSM426497.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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