|
| Status |
Public on Jul 15, 2020 |
| Title |
healthy_1 |
| Sample type |
RNA |
| |
|
| Source name |
iPSC-derived neuronal cultures
|
| Organism |
Homo sapiens |
| Characteristics |
disease: healthy control
|
| Treatment protocol |
iPSC-derived neurons were differentiated for six weeks and then stimulated with 500 nM of oxidized Aß-S8C dimers for 72 h
|
| Growth protocol |
https://doi.org/10.1101/652446
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using the Direct-zol RNA MiniPrep Kit (Zymo Research, CA, USA) according to provider guidelines. After checking the quality of mRNA, 500 ng of RNA were used for complementary DNA synthesized with the TaqMan Reverse Transcription Kit (Applied Biosystems).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Standard Affymetrix hybridization protocol
|
| Scan protocol |
Standard Affymetrix scanning protocol for PrimeView Human Gene Expression Array
|
| Description |
replicate 1
|
| Data processing |
The data were normalised using RMA normalisation from R/Bioconductor
|
| |
|
| Submission date |
Jan 20, 2020 |
| Last update date |
Jul 15, 2020 |
| Contact name |
Wasco Wruck |
| E-mail(s) |
[email protected]
|
| Organization name |
Universitätsklinikum Düsseldorf
|
| Street address |
Moorenstr. 5
|
| City |
Düsseldorf |
| ZIP/Postal code |
40225 |
| Country |
Germany |
| |
|
| Platform ID |
GPL16043 |
| Series (1) |
| GSE143951 |
IPSC-derived neuronal cultures expressing the Alzheimer's disease associated rare TREM2 R47H variant enables the construction of an Aβ-induced gene regulatory network |
|
