|
| Status |
Public on Oct 13, 2020 |
| Title |
sRNA_seq_AGO_IP_PrimaryPolyp_AGO1_I |
| Sample type |
SRA |
| |
|
| Source name |
RNA extracted from immunoprecipitated AGO
|
| Organism |
Nematostella vectensis |
| Characteristics |
developmental stage: Primary polyp
antibody: AGO
|
| Extracted molecule |
total RNA |
| Extraction protocol |
sRNA-seq-Zamore-protocol:Small RNA libraries were constructed as described in Phillip Zamore Lab Illumina TruSeq Small RNA Cloning Protocol April, 2014 (http://www.umassmed.edu/zamore/resources/protocols/). sRNA-seq-modified-nebnext was carried out using NEBNext Multiplex Small RNA Library Prep Set for Illumina kit (cat. no E7300, New Enagland Biolabs) according to the protocol described by: Plotnikova et al. Plant Cell 2019. mRNA sequensing was carried out with Lexogen Sense kit according to the manufacturer's instructions.
|
| |
|
| Library strategy |
RIP-Seq |
| Library source |
transcriptomic |
| Library selection |
other |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
small RNA
sRNA-seq-protocol-modified-nebnext
|
| Data processing |
The data were pre-processed to remove the adapters using cutadapt
processed reads were analysed using miRDeep2 software
The gene models can be found here: https://figshare.com/articles/Nematostella_vectensis_transcriptome_and_gene_models_v2_0/807696
Genome_build: ASM20922v1, INSDC Assembly GCA_000209225.1, Sep 2007.
Supplementary_files_format_and_content: read counts
Supplementary_files_format_and_content: counts.tabular_tabular
|
| |
|
| Submission date |
Jan 24, 2020 |
| Last update date |
Oct 13, 2020 |
| Contact name |
Arie Fridrich |
| E-mail(s) |
[email protected]
|
| Phone |
0586512345
|
| Organization name |
The Hebrew University of Jerusalem
|
| Department |
EEB
|
| Lab |
Yehu Moran
|
| Street address |
1
|
| City |
Maale-AdumimJerusalem |
| State/province |
Israel |
| ZIP/Postal code |
1 |
| Country |
Israel |
| |
|
| Platform ID |
GPL23029 |
| Series (1) |
| GSE144203 |
Unravelling the developmental and functional significance of an ancient Argonaute duplication |
|
| Relations |
| BioSample |
SAMN13916255 |
| SRA |
SRX7626702 |
