|
Status |
Public on Dec 01, 2024 |
Title |
G2300LC |
Sample type |
RNA |
|
|
Source name |
yeast cell culture
|
Organism |
Saccharomyces cerevisiae |
Characteristics |
genotype/variation: flo11 knockout growth time: 6 hrs nutrient composition: SD growth medium: liquid
|
Treatment protocol |
quick-frozen in acid phenol
|
Growth protocol |
6 hour growth
|
Extracted molecule |
total RNA |
Extraction protocol |
Qiagen RNeasy mini-kit
|
Label |
biotin
|
Label protocol |
Affymetrix IVT labeling
|
|
|
Hybridization protocol |
Following fragmentation, ten micrograms of adjusted cRNA fromeach sample was hybridized for 16 hours at 45 deg C to Affymetrix GeneChip
|
Scan protocol |
Scanning was performed using the Affymetrix GeneChip 3000 Scanner. Images were processed into CEL files with the Affymetrix GCOS software.
|
Description |
flo11 knockout, SD liquid C
|
Data processing |
Raw CEL intensity data were RMA normalized using R/Bioconductor
|
|
|
Submission date |
Jul 23, 2009 |
Last update date |
Dec 01, 2024 |
Contact name |
Gregory Carter |
E-mail(s) |
[email protected]
|
Phone |
1-207-288-6025
|
Organization name |
The Jackson Laboratory
|
Street address |
600 Main Street
|
City |
Bar Harbor |
State/province |
ME |
ZIP/Postal code |
04609 |
Country |
USA |
|
|
Platform ID |
GPL2529 |
Series (1) |
GSE17295 |
Yeast Thigmotropism Controlled by a Cell-Surface Mucin and a MAP-Kinase Pathway |
|