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Sample GSM435066 Query DataSets for GSM435066
Status Public on Feb 01, 2010
Title FrontalC_HIVMDD_2
Sample type RNA
 
Source name Frozen_frontal_Cortex_HIVMDD
Organism Homo sapiens
Characteristics tissue: Brain, frontal cortex
latest mdd visit: 12 mo
age: 38
hiv status: HIV+
gender: Male
pmi: 12
Extracted molecule total RNA
Extraction protocol Trizol extraction per manufacturer's protocols.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Gene expression data from frontal cortex of patient CA143
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date Jul 30, 2009
Last update date Aug 28, 2018
Contact name Erick T Tatro
E-mail(s) [email protected]
Phone 858-246-0653
Fax 858-534-4484
Organization name University of California San Diego
Department Psychiatry
Lab Tatro
Street address 9500 Gilman Dr
City La Jolla
State/province CA
ZIP/Postal code 92093-0603
Country USA
 
Platform ID GPL570
Series (2)
GSE17440 Gene Expression in Frontal Cortex in Major Depression and HIV
GSE17491 Evidence for Alteration of Gene Regulatory Networks through MicroRNAs of the HIV-Infected Brain
Relations
Reanalyzed by GSE119087

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 4871.4
AFFX-BioB-M_at 5134.3
AFFX-BioB-3_at 3717.7
AFFX-BioC-5_at 14512.3
AFFX-BioC-3_at 17904.4
AFFX-BioDn-5_at 27830.7
AFFX-BioDn-3_at 72883.7
AFFX-CreX-5_at 142501.9
AFFX-CreX-3_at 154023.3
AFFX-DapX-5_at 368
AFFX-DapX-M_at 210.5
AFFX-DapX-3_at 34.2
AFFX-LysX-5_at 209.3
AFFX-LysX-M_at 310.8
AFFX-LysX-3_at 278.2
AFFX-PheX-5_at 49.3
AFFX-PheX-M_at 48.3
AFFX-PheX-3_at 206
AFFX-ThrX-5_at 129.7
AFFX-ThrX-M_at 266.4

Total number of rows: 54675

Table truncated, full table size 911 Kbytes.




Supplementary file Size Download File type/resource
GSM435066.CEL.gz 5.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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