|
| Status |
Public on Feb 01, 2010 |
| Title |
FrontalC_HIV_1 |
| Sample type |
RNA |
| |
|
| Source name |
Frozen_frontal_cortex_HIV
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Brain, frontal cortex
latest mdd visit: NO
age: 54
hiv status: HIV+
gender: Male
pmi: 12
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction per manufacturer's protocols.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from frontal cortex of patient CC103
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Jul 30, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Erick T Tatro |
| E-mail(s) |
[email protected]
|
| Phone |
858-246-0653
|
| Fax |
858-534-4484
|
| Organization name |
University of California San Diego
|
| Department |
Psychiatry
|
| Lab |
Tatro
|
| Street address |
9500 Gilman Dr
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093-0603 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE17440 |
Gene Expression in Frontal Cortex in Major Depression and HIV |
| GSE17491 |
Evidence for Alteration of Gene Regulatory Networks through MicroRNAs of the HIV-Infected Brain |
|
| Relations |
| Reanalyzed by |
GSE119087 |
