|
| Status |
Public on Aug 10, 2009 |
| Title |
Lung dnCREB-PM replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
Lung
|
| Organism |
Mus musculus |
| Characteristics |
strain: CD-1
tissue: lung
age: 20 week
genotype: dominant negative CREB1 driven by cardiomyocyte-specific myosin heavy chain-alpha promoter
|
| Treatment protocol |
20mg/kg 1.9µm particulate matter, lung harvest after 36 hour
|
| Growth protocol |
standard conditions (12h light-dark cycle, 25-27ºC, ~40% humidity)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse320_2. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Mouse, male
|
| Data processing |
Normalized using GCRMA
|
| |
|
| Submission date |
Aug 03, 2009 |
| Last update date |
Oct 01, 2009 |
| Contact name |
Yong Huang |
| E-mail(s) |
[email protected]
|
| Phone |
(434) 243-0842
|
| Organization name |
University of Viginia
|
| Department |
Medicine
|
| Street address |
1340 Jefferson Park Ave
|
| City |
Charlottesville |
| State/province |
VA |
| ZIP/Postal code |
22908 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE17478 |
Particulate Matter effect on Mouse Model of Cardiac Failure: Lung and Heart Left Ventricle |
|
