|
| Status |
Public on Feb 01, 2010 |
| Title |
frontal_cortex_control |
| Sample type |
RNA |
| |
|
| Source name |
brain_frontal_frozen_control
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: brain
brain region: frontal cortex
mdd-status: Negative
hiv-status: Negative
|
| Treatment protocol |
not applicable
|
| Growth protocol |
not applicable
|
| Extracted molecule |
total RNA |
| Extraction protocol |
MirVana (Applied Biosystems) per manufacturer's protocols of frozen tissue.
|
| Label |
FAM
|
| Label protocol |
not applicable
|
| |
|
| Hybridization protocol |
not applicable
|
| Scan protocol |
not applicable
|
| Description |
Pooled RNA samples from 3 subjects, average delta CT from technical triplicates reported in data table, each raw data file is from 1 technical triplicate.
Standard Taqman Assay: Quantitative real-time PCR was performed using Applied Biosystems 7900HT system and universal cycling conditions (10 min. at 95C; 15 sec. at 95C; 1 min. at 60C for 40 cycles), FAM channel was measured.
|
| Data processing |
Data for table was processed using SDS 2.3 software (Applied Biosystems, Foster City, CA) and importing files to RQ (relative quantification) Manager 2.0 software (Applied Biosystems). Normalized by subtracting RNU44-CT to get Delta CT. Average Delta CT from technical triplicates is reported in data table, raw data (*.txt, *.sds) supplied as supplementary files (*.sds readable in SDS and RQ manager software).
|
| |
|
| Submission date |
Aug 03, 2009 |
| Last update date |
Dec 21, 2009 |
| Contact name |
Erick T Tatro |
| E-mail(s) |
[email protected]
|
| Phone |
858-246-0653
|
| Fax |
858-534-4484
|
| Organization name |
University of California San Diego
|
| Department |
Psychiatry
|
| Lab |
Tatro
|
| Street address |
9500 Gilman Dr
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093-0603 |
| Country |
USA |
| |
|
| Platform ID |
GPL8979 |
| Series (2) |
| GSE17486 |
MicroRNAs in the HIV and Major Depressive Brain |
| GSE17491 |
Evidence for Alteration of Gene Regulatory Networks through MicroRNAs of the HIV-Infected Brain |
|
