|
| Status |
Public on Mar 30, 2020 |
| Title |
65_Vehicle |
| Sample type |
RNA |
| |
|
| Source name |
primary cultured airway smooth muscle cell
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: primary cultured airway smooth muscle cell
donor: 65
treatment: Vehicle
Sex: Male
age: 65
asthma: Yes
race: EuropeanAmerican
|
| Treatment protocol |
ASMCs were exposed to IL-13 (10ng/mL), IL-17 (3 ng/mL), IL-13+IL-17, or vehicle (10% FBS in PBS) for 24 hours prior to RNA and DNA extraction.
|
| Growth protocol |
ASMCs were cultured in 96-well Nusil coated plates in serum free media for 48 hours prior to cytokine exposure.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA for gene expression studies were isolated from cell lysates using the QIAgen AllPrep Kit.
|
| Label |
biotin
|
| Label protocol |
not provided
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Standard Illumina scan protocol.
|
| Description |
Plt1_C5
|
| Data processing |
All samples had RIN scores >9.6 The probe level raw intensity values across arrays were normalized using quantile normalization and background corrected normalized expression values were obtained for each probe using the R package lumi. Probes that were indistinguishable from background intensity (P<0.01), contained more than one HapMap SNP, or mapped to multiple locations in the genome were excluded. When multiple probes mapped to the same gene, the median probe expression value was used for that gene.
|
| |
|
| Submission date |
Mar 04, 2020 |
| Last update date |
Mar 30, 2020 |
| Contact name |
Emma Thompson |
| E-mail(s) |
[email protected], [email protected]
|
| Organization name |
The University of Chicago
|
| Department |
Human Genetics
|
| Street address |
920 E 58th St, CLSC Rm 501
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60637 |
| Country |
USA |
| |
|
| Platform ID |
GPL28226 |
| Series (2) |
| GSE146374 |
Genome-wide gene expression study in primary cultured airway smooth muscle cells |
| GSE146377 |
Genome-wide gene expression and methylation studies in primary cultured airway smooth muscle cells |
|
