|
| Status |
Public on Sep 30, 2009 |
| Title |
SS patient number 43; diagnostic sample |
| Sample type |
genomic |
| |
|
| Source name |
SS patient peripheral blood, CD3+ T lymphocytes
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: peripheral blood
cell type: CD3+ T lymphocytes
disease: Sézary Syndrome
tumour type: Cutaneous T cell lymphoma
|
| Treatment protocol |
Mononuclear cells were isolated from peripheral blood of patients with SS and either cryopreserved in liquid nitrogen or used immediately for CD3 positive selection and DNA extraction. Granulocyte fractions were separately collected, freezed or used immediately for DNA extraction.
|
| Growth protocol |
Not Applicable.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was prepared using standard proteinase K digestion and phenol/chloroform extraction.
|
| Label |
Biotin
|
| Label protocol |
Affymetrix 10K mapping assay protocol.
|
| |
|
| Hybridization protocol |
Affymetrix 10K mapping assay protocol.
|
| Scan protocol |
Affymetrix GeneChip scanner 3000-7G.
|
| Description |
Affymetrix 10K SNP genotype data
P43T
|
| Data processing |
GCOS, GTYPE. Genotype calling algorithm MPAM.
|
| |
|
| Submission date |
Aug 11, 2009 |
| Last update date |
May 04, 2016 |
| Contact name |
Elisabetta Caprini |
| E-mail(s) |
[email protected]
|
| Organization name |
IDI-IRCCS
|
| Street address |
Via dei Monti di Creta 104
|
| City |
Roma |
| State/province |
Italy |
| ZIP/Postal code |
00167 |
| Country |
Italy |
| |
|
| Platform ID |
GPL2641 |
| Series (2) |
| GSE17595 |
Affymetrix SNP array data for Sézary Syndrome (SS) samples |
| GSE17602 |
Identification of regions and genes important in Sézary syndrome pathogenesis using genomic and expression microarrays |
|
