|
| Status |
Public on Aug 31, 2009 |
| Title |
IP ZT3, replica 2 |
| Sample type |
RNA |
| |
|
| Source name |
fly head
|
| Organism |
Drosophila melanogaster |
| Characteristics |
time point: ZT3
replicate: 2
tissue: head
developmental stage: adult
|
| Growth protocol |
Flies were grown under standard conditions in 12hs light and 12hs dark cycles
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Heads were collected and RNA extracted using TRIZOL
|
| Label |
biotin
|
| Label protocol |
RNA was utilized to prepare cDNA and cRNA using the Affymetrix IVT labeling Kit
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 18 hrs at 45C on GeneChip Drosophila Genome 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned with the Affymetrix GeneArray Scanner G7
|
| Description |
IP sample, ZT3
|
| Data processing |
The data were processed with GCRMA algorithm in the Bioconductor with default settings
|
| |
|
| Submission date |
Aug 13, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Ken Sugino |
| Organization name |
Janelia Research Campus
|
| Lab |
NeuroSeq
|
| Street address |
19700 Helix Dr
|
| City |
Ashburn |
| State/province |
VA |
| ZIP/Postal code |
20147 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (2) |
| GSE17627 |
AGO 1 IMMUNOPRECIPITATION MICROARRAYS |
| GSE17629 |
Circadian analysis of miRNAs and their targets |
|
| Relations |
| Reanalyzed by |
GSE119084 |
